Dr Helena AzevedoMEng, PhD
Senior Lecturer in Biomedical Engineering & Biomaterials
Advances in self-assembly offer new opportunities in molecular design of biomaterials. A major advantage of these systems is their ability to generate similar fibrillar structures to those found in the natural extracellular matrix (ECM) of tissues and to integrate directly in the structure biomolecular ligands for cell signaling.
Dr Helena Azevedo works at the interface of chemistry, materials science, nanotechnology, self-assembly, biomaterials and medicine. In particular, she works with self-assembling peptides (design, synthesis and characterization) and their combination with macromolecules (natural and synthetic polymers) to develop nanostructured biomaterials (microcapsules, membranes, hydrogels) with potential applications in the regeneration of cartilage and skin tissues. Within these studies, she is interested in using supramolecular chemistry to program molecules for self-assembly into structures with bioactivity and to understand the energy pathways for their self-assembly into functional macroscopic biomaterials.
Shi Y, Hu Y, Ochbaum G, Lin R, Bitton R, Cui H and Azevedo HS (2017). Enzymatic activation of cell-penetrating peptides in self-assembled nanostructures triggers fibre-to-micelle morphological transition. Chem Commun (Camb) 10.1039/c7cc03512h
MATA A, Azevedo HS, Botto L, Gavara N and Su L (2017). New Bioengineering Breakthroughs and Enabling Tools in Regenerative Medicine. Current Stem Cell Reports 10.1007/s40778-017-0081-9
Martins IM, Reis RL and Azevedo HS (2016). Phage Display Technology in Biomaterials Engineering: Progress and Opportunities for Applications in Regenerative Medicine. Acs Chem Biol 10.1021/acschembio.5b00717
Inostroza-Brito KE, Collin E, Siton-Mendelson O, Smith KH, Monge-Marcet A, Ferreira DS, Rodríguez RP, Alonso M, Rodríguez-Cabello JC, Reis RL, Sagués F, Botto L, Bitton R, Azevedo HS and Mata A (2015). Co-assembly, spatiotemporal control and morphogenesis of a hybrid protein-peptide system. Nat Chem vol. 7, (11) 897-904. 10.1038/nchem.2349
Azevedo HS and Pashkuleva I (2015). Biomimetic supramolecular designs for the controlled release of growth factors in bone regeneration. Adv Drug Deliv Rev vol. 94, 63-76. 10.1016/j.addr.2015.08.003
Balmayor ER, Baran TE, Unger M, Marques AP, Azevedo HS and Reis RL (2015). Presence of starch enhances in vitro biodegradation and biocompatibility of a gentamicin delivery formulation. J Biomed Mater Res B Appl Biomater vol. 103, (8) 1610-1620. 10.1002/jbm.b.33343
Conde J, Bao C, Tan Y, Cui D, Edelman ER, Azevedo HS, Byrne HJ, Artzi N and Tian F (2015). Dual targeted immunotherapy via in vivo delivery of biohybrid RNAi-peptide nanoparticles to tumour-associated macrophages and cancer cells. Adv Funct Mater vol. 25, (27) 4183-4194. 10.1002/adfm.201501283
Ferreira DS, Lin Y-A, Cui H, Hubbell JA, Reis RL and Azevedo HS (2015). Molecularly engineered self-assembling membranes for cell-mediated degradation. Adv Healthc Mater vol. 4, (4) 602-612. 10.1002/adhm.201400586
Pereira RC, Costa-Pinto AR, Frias AM, Neves NM, Azevedo HS and Reis RL (2015). In vitro chondrogenic commitment of human Wharton's jelly stem cells by co-culture with human articular chondrocytes. Journal of Tissue Engineering and Regenerative Medicine n/a-n/a. 10.1002/term.2085
Susano MA, Leonor IB, Reis RL and Azevedo HS (2014). Elastic biodegradable starch/ethylene-co-vinyl alcohol fibre-mesh scaffolds for tissue engineering applications. Journal of Applied Polymer Science vol. 131, (14) 10.1002/app.40504
Mendes AC, Baran ET, Reis RL and Azevedo HS (2013). Self-assembly in nature: Using the principles of nature to create complex nanobiomaterials. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology vol. 5, (6) 582-612. 10.1002/wnan.1238
Ferreira DS, Reis RL and Azevedo HS (2013). Peptide-based microcapsules obtained by self-assembly and microfluidics as controlled environments for cell culture. Soft Matter vol. 9, (38) 9237-9248. 10.1039/c3sm51189h
Ferreira DS, Marques AP, Reis RL and Azevedo HS (2013). Hyaluronan and self-assembling peptides as building blocks to reconstruct the extracellular environment in skin tissue. Biomaterials Science vol. 1, (9) 952-964. 10.1039/c3bm60019j
Mendes AC, Baran ET, Reis RL and Azevedo HS (2013). Fabrication of phospholipid-xanthan microcapsules by combining microfluidics with self-assembly. Acta Biomaterialia vol. 9, (5) 6675-6685. 10.1016/j.actbio.2013.01.035
Mendes AC, Smith KH, Tejeda-Montes E, Engel E, Reis RL, Azevedo HS and Mata A (2013). Co-assembled and microfabricated bioactive membranes. Advanced Functional Materials vol. 23, (4) 430-438. 10.1002/adfm.201201065
Alves NM, Leonor IB, Azevedo HS, Reis RL and Mano JF (2012). Surfaces Inducing Biomineralization. 10.1002/9783527652273.ch15
Mendes AC, Baran ET, Lisboa P, Reis RL and Azevedo HS (2012). Microfluidic fabrication of self-assembled peptide-polysaccharide microcapsules as 3D environments for cell culture. Biomacromolecules vol. 13, (12) 4039-4048. 10.1021/bm301332z
Smith KH, Inostroza KE, Alonso M, Reis RL, Azevedo HS and Mata A (2012). Spontaneous co-assembly of molecularly designed building blocks into hierarchically ordered, dynamic, hybrid macrotubes. Journal of Tissue Engineering and Regenerative Medicine vol. 6, 224-225.
Mendes AC, Baran ET, Pereira RC, Azevedo HS and Reis RL (2012). Encapsulation and Survival of a Chondrocyte Cell Line within Xanthan Gum Derivative. Macromolecular Bioscience vol. 12, (3) 350-359. 10.1002/mabi.201100304
Balmayor ER, Baran ET, Azevedo HS and Reis RL (2012). Injectable biodegradable starch/chitosan delivery system for the sustained release of gentamicin to treat bone infections. Carbohydrate Polymers vol. 87, (1) 32-39. 10.1016/j.carbpol.2011.06.078
Susano M, Frias AM, Leonor IB, Reis RL and Azevedo HS (2011). Biodegradable fiber-mesh scaffolds with elastic properties and their potential use in tissue engineering. 24th European Conference On Biomaterials - Annual Conference of The European Society For Biomaterials
Mendes AC, Baran ET, Nunes C, Coimbra MA, Azevedo HS and Reis RL (2011). Palmitoylation of xanthan polysaccharide for self-assembly microcapsule formation and encapsulation of cells in physiological conditions. Soft Matter vol. 7, (20) 9647-9658. 10.1039/c1sm05594a
Pereira RC, Scaranari M, Gentili C, Cancedda R, Frias AM, Reis RL and Azevedo HS (2011). HYALURONIC ACID MICROPARTICLES AS INJECTABLE DRUG CARRIER FOR KNEE CARTILAGE REPAIR: EFFECT ON ARTICULAR CHONDROCYTE BEHAVIOR. International Journal of Artificial Organs vol. 34, (8) 667-667.
Mendes AC, Baran ET, Azevedo HS and Reis RL (2011). MICROFLUIDIC ENCAPSULATION OF CELLS INTO SELF-ASSEMBLING XANTHAN-PHOSPOLIPID AMPHIPHILE FOR CELL THERAPY. International Journal of Artificial Organs vol. 34, (8) 623-623.
Balmayor ER, Pashkuleva I, Frias AM, Azevedo HS and Reis RL (2011). Synthesis and functionalization of superparamagnetic poly-?- caprolactone microparticles for the selective isolation of subpopulations of human adipose-derived stem cells. Journal of The Royal Society Interface vol. 8, (59) 896-905. 10.1098/rsif.2010.0531
Balmayor ER, Azevedo HS and Reis RL (2011). Controlled delivery systems: From pharmaceuticals to cells and genes. Pharmaceutical Research vol. 28, (6) 1241-1258. 10.1007/s11095-011-0392-y
Pereira RC, Gentili C, Cancedda R, Azevedo HS and Reis RL (2011). Encapsulation of human articular chondrocytes into 3D hydrogel: phenotype and genotype characterization. Methods in Molecular Biology (Clifton, N.J.) vol. 695, 167-181. 10.1007/978-1-60761-984-0_11
Pashkuleva I, López-Pérez PM, Azevedo HS and Reis RL (2010). Highly porous and interconnected starch-based scaffolds: Production, characterization and surface modification. Materials Science and Engineering C vol. 30, (7) 981-989. 10.1016/j.msec.2010.04.019
Bessa PC, Balmayor ER, Azevedo HS, Nürnberger S, Casal M, Van Griensven M, Reis RL and Redl H (2010). Silk fibroin microparticles as carriers for delivery of human recombinant BMPs. Physical characterization and drug release. Journal of Tissue Engineering and Regenerative Medicine vol. 4, (5) 349-355. 10.1002/term.245
Alves NM, Leonor IB, Azevedo HS, Reis RL and Mano JF (2010). Designing biomaterials based on biomineralization of bone. Journal of Materials Chemistry vol. 20, (15) 2911-2921. 10.1039/b910960a
Balmayor ER, Feichtinger GA, Azevedo HS, Van Griensven M and Reis RL (2009). Starch-poly-?-caprolactone microparticles reduce the needed amount of BMP-2. Clinical Orthopaedics and Related Research vol. 467, (12) 3138-3148. 10.1007/s11999-009-0954-z
Pereira RC, Scaranari M, Castagnola P, Grandizio M, Azevedo HS, Reis RL, Cancedda R and Gentili C (2009). Novel injectable gel (system) as a vehicle for human articular chondrocytes in cartilage tissue regeneration. Journal of Tissue Engineering and Regenerative Medicine vol. 3, (2) 97-106. 10.1002/term.145
Martins AM, Pereira RC, Leonor IB, Azevedo HS and Reis RL (2009). Chitosan scaffolds incorporating lysozyme into CaP coatings produced by a biomimetic route: A novel concept for tissue engineering combining a self-regulated degradation system with in situ pore formation. Acta Biomaterialia vol. 5, (9) 3328-3336. 10.1016/j.actbio.2009.05.027
Azevedo HS and Reis RL (2009). Encapsulation of ?-amylase into starch-based biomaterials: An enzymatic approach to tailor their degradation rate. Acta Biomaterialia vol. 5, (8) 3021-3030. 10.1016/j.actbio.2009.04.039
Balmayor ER, Tuzlakoglu K, Azevedo HS and Reis RL (2009). Preparation and characterization of starch-poly-?-caprolactone microparticles incorporating bioactive agents for drug delivery and tissue engineering applications. Acta Biomaterialia vol. 5, (4) 1035-1045. 10.1016/j.actbio.2008.11.006
Leonor IB, Alves CM, Azevedo HS and Reis RL (2009). Effects of protein incorporation on calcium phosphate coating. Materials Science and Engineering C vol. 29, (3) 913-918. 10.1016/j.msec.2008.08.003
Capito RM, Azevedo HS, Velichko Y, Mata A and Stupp SI (2008). Self-assembly of biomaterials at an interface: Formation of sacs, membranes, and strings. 8th World Biomaterials Congress 2008 vol. 1,
Gomes M, Azevedo H, Malafaya P, Silva S, Oliveira J, Silva G, Sousa R, Mano J and Reis R (2008). Natural Polymers in Tissue Engineering Applications. 10.1016/B978-0-12-370869-4.00006-9
Pereira R, Scaranari M, Castagnola P, Grandizio M, Azevedo H, Reis RL, Cancedda R and Gentili C (2008). Novel injectable gel system as a vehicle for human articular chondrocytes encapsulation for cartilage tissue regeneration. 8th World Biomaterials Congress 2008 vol. 3,
Balmayor ER, Baran ET, Azevedo HS and Reis RL (2008). Highly biodegradable starch-conjugated chitosan microspheres as carriers for the controlled release of aminoglycosides antibiotic drugs. 8th World Biomaterials Congress 2008 vol. 1,
Martins AM, Santos MI, Azevedo HS, Malafaya PB and Reis RL (2008). Natural origin scaffolds with in situ pore forming capability for bone tissue engineering applications. Acta Biomater vol. 4, (6) 1637-1645. 10.1016/j.actbio.2008.06.004
Reis RL, Neves NM, Mano JF, Gomes ME, Marques AP and Azevedo HS (2008). Preface. Natural-Based Polymers For Biomedical Applications 10.1016/B978-1-84569-264-3.50034-2
Azevedo HS, Santos TC and Reis RL (2008). Controlling the degradation of natural polymers for biomedical applications. 10.1533/9781845694814.1.106
Gomes ME, Azevedo HS, Moreira AR, Ellä V, Kellomäki M and Reis RL (2008). Starch-poly(?-caprolactone) and starch-poly(lactic acid) fibre-mesh scaffolds for bone tissue engineering applications: Structure, mechanical properties and degradation behaviour. Journal of Tissue Engineering and Regenerative Medicine vol. 2, (5) 243-252. 10.1002/term.89
Balmayor ER, Tuzlakoglu K, Marques AP, Azevedo HS and Reis RL (2008). A novel enzymatically-mediated drug delivery carrier for bone tissue engineering applications: Combining biodegradable starch-based microparticles and differentiation agents. Journal of Materials Science: Materials in Medicine vol. 19, (4) 1617-1623. 10.1007/s10856-008-3378-5
Capito RM, Azevedo HS, Velichko YS, Mata A and Stupp SI (2008). Self-assembly of large and small molecules into hierarchically ordered sacs and membranes. Science vol. 319, (5871) 1812-1816. 10.1126/science.1154586
Pashkuleva I, Azevedo HS and Reis RL (2008). Surface structural investigation of starch-based biomaterials. Macromolecular Bioscience vol. 8, (2) 210-219. 10.1002/mabi.200700076
Reis RL, Neves NM, Mano JF, Gomes ME, Marques AP and Azevedo HS (2008). Natural-based polymers for biomedical applications. Natural-Based Polymers For Biomedical Applications 1-802. 10.1533/9781845694814
Mano JF, Silva GA, Azevedo HS, Malafaya PB, Sousa RA, Silva SS, Boesel LF, Oliveira JM, Santos TC, Marques AP, Neves NM and Reis RL (2007). Natural origin biodegradable systems in tissue engineering and regenerative medicine: Present status and some moving trends. Journal of The Royal Society Interface vol. 4, (17) 999-1030. 10.1098/rsif.2007.0220
Boesel LF, Azevedo HS and Reis RL (2006). Incorporation of ?-amylase enzyme and a bioactive filler into hydrophilic, partially degradable, and bioactive cements (HDBCs) as a new approach to tailor simultaneously their degradation and bioactive behavior. Biomacromolecules vol. 7, (9) 2600-2609. 10.1021/bm060387j
Azevedo HS, Leonor IB and Reis RL (2006). Incorporation of proteins with different isoelectric points into biomimetic Ca-P coatings: A new approach to produce hybrid coatings with tailored properties. Key Engineering Materials vol. 309-311 II, 755-758.
Azevedo HS, Leonor IB, Alves CM and Reis RL (2005). Incorporation of proteins and enzymes at different stages of the preparation of calcium phosphate coatings on a degradable substrate by a biomimetic methodology. Materials Science and Engineering C vol. 25, (2) 169-179. 10.1016/j.msec.2005.01.007
Boesel LF, Azevedo HS and Reis RL (2004). Novel hydrophilic, partially degradable and bioactive bone cements containing starch and a bioactive glass. Transactions - 7th World Biomaterials Congress
Azevedo HS and Reis RL (2004). Effect of incubation conditions and enzyme cocktails on the in vitro degradation behaviour of different starch-based biomaterials. Transactions - 7th World Biomaterials Congress
Martins AM, Malafaya PB, Azevedo HS, Santos MI, Coutinho OP and Reis RL (2004). Natural origin scaffolds with in situ gradual pore forming capability: Development and characterization. Transactions - 7th World Biomaterials Congress
Azevedo HS, Leonor IB, Alves CM, Goldsmith RJ and Reis RL (2004). Influence of protein incorporation in the nucleation and growth of biomimetic calcium phosphate coatings. Transactions - 7th World Biomaterials Congress
Azevedo HS, Gama FM and Reis RL (2003). In vitro assessment of the enzymatic degradation of several starch based biomaterials. Biomacromolecules vol. 4, (6) 1703-1712. 10.1021/bm0300397
Leonor IB, Azevedo HS, Alves CM and Reis RL (2003). Effects of the incorporation of proteins and active enzymes on biomimetic calcium-phosphate coatings. Key Engineering Materials vol. 240-242, 97-100.
Oliveira AL, Leonor IB, Malafaya PB, Alves CM, Azevedo HS and Reis RL (2003). Tailoring the bioactivity of natural origin inorganic - Polymeric based systems. Key Engineering Materials vol. 240-242, 111-142.
Azevedo H, Bishop D and Cavaco-Paulo A (2002). Possibilities for recycling cellulases after use in cotton processing: Part I: Effects of end-product inhibition, thermal and mechanical deactivation, and cellulase depletion by adsorption. Applied Biochemistry and Biotechnology - Part a Enzyme Engineering and Biotechnology vol. 101, (1) 61-75. 10.1385/ABAB:101:1:61
Azevedo H, Bishop D and Cavaco-Paulo A (2002). Possibilities for recycling cellulases after use in cotton processing: Part II: Separation of cellulases from reaction products and released dyestuffs by ultrafiltration. Applied Biochemistry and Biotechnology - Part a Enzyme Engineering and Biotechnology vol. 101, (1) 77-91. 10.1385/ABAB:101:1:77
Azevedo H, Pereira Ramos L and Cavaco-Paulo A (2001). Desorption of cellulases from cotton powder. Biotechnology Letters vol. 23, (17) 1445-1448. 10.1023/A:1011619425613
Azevedo H and Cavaco-Paulo A (2001). Recovery of cellulases after textile processing. Abstracts of Papers of The American Chemical Society vol. 221, U176-U176.
Azevedo H, Bishop D and Cavaco-Paulo A (2000). Effects of agitation level on the adsorption, desorption, and activities on cotton fabrics of full length and core domains of EGV (Humicola insolens) and CenA (Cellulomonas fimi). Enzyme and Microbial Technology vol. 27, (3-5) 325-329. 10.1016/S0141-0229(00)00205-2
Andreaus J, Azevedo H and Cavaco-Paulo A (1999). Effects of temperature on the cellulose binding ability of cellulase enzymes. Journal of Molecular Catalysis - B Enzymatic vol. 7, (1-4) 233-239. 10.1016/S1381-1177(99)00032-6